A Low-Cost Closed-Tube Method for Detection of Adulteration in Ground Meat

The increased public concern about adulteration and mislabelling of meat products has fueled a need for the development fast, reliable, cost-effective species detection methods. Hence, we developed high resolution melt analysis (HRMA) discrimination commonly used in industry their possible adulterants. A universal primer pair spanning ⁓238 bp fragment mitochondrial 16S rRNA gene was designed to perform HRMA. workflow validated on standard samples, ternary mixtures eight different species, 56 commercial samples. Distinct melting profiles were generated each several mixtures, food assay sufficiently sensitive detect 0.003 ng/µl DNA every targeted species. In short, HRM is rapid, cost-effective, efficient system confirmation origin.